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mouse antimap2  (Millipore)


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    Structured Review

    Millipore mouse antimap2
    Mouse Antimap2, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+antimap2/anti+map2/pm39139398-47-10-13
    Average 90 stars, based on 1 article reviews
    mouse antimap2 - by Bioz Stars, 2026-09
    90/100 stars

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    Incubation:

    Article Title: Licochalcone A attenuates NMDA-induced neurotoxicity.
    Article Snippet: Permeabilization was achieved using 0.3% Triton X-100 for 5 minutes, followed by blocking with 5% Bovine Serum Albumin (BSA, Sigma Aldrich, USA) for 90 minutes at room temperature (Yun et al. 2023). .. Overnight incubation at 4°C was done with primary antibodies including mouse antiMAP2 (1:200, Sigma-Aldrich, USA), rabbit anti-PSD95 (1:200, Invitrogen, USA), mouse anti-GFAP (1:200, Sigma-Aldrich, USA), rabbit anti-IBA-1 (1:500, Wako, Japan) and mouse anti-TNF-α (1:200, Santa Cruz, USA). .. This is followed by incubation with Alexa Fluor 488 anti-rabbit IgG (1:500, Invitrogen, USA), and Alexa Fluor 555 anti-mouse IgG (1:1000, Invitrogen, USA) secondary antibodies.

    Article Title: Spatial gene's (Tbata) implication in neurite outgrowth and dendrite patterning in hippocampal neurons.
    Article Snippet: Proteinswere visualized using horseradish peroxidase-conjugated secondary antibody (1:1000; Amersham Pharmacia Biotech) and the enhanced chemiluminescence detection system (Pierce). .. Immunofluorescence Cells were post-fixed in 4% PFA, permeabilized with 0.1% Triton, blocked and incubated with rabbit anti-Spatial (1:100), mouse antiMAP2 (1:500; clone AP20; Millipore), antiphosphorylated NF-H (1:900, clone SMI31, Chemicon) or rat anti-GFP (1:500) antibodies. .. They were then incubated with Alexa fluorescent secondary antibodies (Molecular Probes).

    Article Title: Hindbrain boundaries as niches of neural progenitor and stem cells regulated by the extracellular matrix proteoglycan chondroitin sulphate.
    Article Snippet: Immunofluorescence Whole mount staining: chick or mouse embryos were harvested at st.18 or E10.5, respectively, cleaned from surrounding membranes and fixed in 4% PFA (Sigma-Aldrich, USA) ON at 4°C. .. Embryos were next washed with PBS, and incubated in blocking buffer (0.1% Tween20 in PBS (PBT) with 5% goat serum) (Biological Industries, Israel) for 2 h. Next, embryos were incubated ON at 4°C in blocking solution with the following primary antibodies; rabbit anti Sox2 (1:400; Millipore, USA), mouse anti-CSPG & mouse antiMap2(1:80 and 1:200, respectively, Sigma-Aldrich, USA), rat anti-DSD-1 (1:200; SigmaAldrich, USA), rabbit anti-FGF8 (1:200; Thermo Fisher Scientific, USA), mouse anti-Tuj1 & mouse anti-HuC/D (both 1:400; Abcam, USA); rabbit anti-MMP2 & rabbit anti-MMP9 (both 1:100, Abcam, USA), mouse anti-Pax2 (1:50, Abcam, USA), goat anti-Doublecortin (1:200, Santa-Cruz Biotechnology, USA), mouse anti-Islt1/Lhx1/5/Laminin-C1/Tag1/HSPG (all .. Following washes, embryos were incubated for 2 h at RT with the following secondary antibodies; Goat anti-mouse Alexa488, Goat anti-mouse Alexa594, Goat anti-rabbit Alexa488, Goat anti-rabbit Alexa594 (1:300; diluted in blocking solution; Life Technologies, USA).

    Article Title: Astroglial exosome HepaCAM signaling and ApoE antagonization coordinates early postnatal cortical pyramidal neuronal axon growth and dendritic spine formation.
    Article Snippet: .. The cells were blocked in3%bovine serumalbumin for 30min and incubated with the following primary antibodies overnight at 4 °C: β-III tubulin (1:1000, MAB1195, R&D system), rabbit anti-MAP2 (1:1000, GeneTex, GTX133109), Gap43 Antibody (1:500, Novus Biologicals, clone 2G13), anti-mouse Tau (1:500, GeneTex), mouse antiMap2 (1:1000, Sigma, M9942), rat anti-GFAP (1:5000, zymed, 273756), rabbit anti-GFAP (1:1000, Dako), and anti-human Tau (1:500, Dako). .. After incubation with the primary antibodies, neurons were washed three times with PBS, and incubated with following secondary antibodies for 1 h at room temperature: anti-mouse Alexa Fluor 488, antirabbit Alexa Fluor 568 and anti-goat 647 Alexa Fluor (1:1000, Invitrogen), and mounted with ProlongTM Glass Antifade Mountant with NucBlueTM Stain (Invitrogen).

    Immunofluorescence:

    Article Title: Spatial gene's (Tbata) implication in neurite outgrowth and dendrite patterning in hippocampal neurons.
    Article Snippet: Proteinswere visualized using horseradish peroxidase-conjugated secondary antibody (1:1000; Amersham Pharmacia Biotech) and the enhanced chemiluminescence detection system (Pierce). .. Immunofluorescence Cells were post-fixed in 4% PFA, permeabilized with 0.1% Triton, blocked and incubated with rabbit anti-Spatial (1:100), mouse antiMAP2 (1:500; clone AP20; Millipore), antiphosphorylated NF-H (1:900, clone SMI31, Chemicon) or rat anti-GFP (1:500) antibodies. .. They were then incubated with Alexa fluorescent secondary antibodies (Molecular Probes).

    Blocking Assay:

    Article Title: Hindbrain boundaries as niches of neural progenitor and stem cells regulated by the extracellular matrix proteoglycan chondroitin sulphate.
    Article Snippet: Immunofluorescence Whole mount staining: chick or mouse embryos were harvested at st.18 or E10.5, respectively, cleaned from surrounding membranes and fixed in 4% PFA (Sigma-Aldrich, USA) ON at 4°C. .. Embryos were next washed with PBS, and incubated in blocking buffer (0.1% Tween20 in PBS (PBT) with 5% goat serum) (Biological Industries, Israel) for 2 h. Next, embryos were incubated ON at 4°C in blocking solution with the following primary antibodies; rabbit anti Sox2 (1:400; Millipore, USA), mouse anti-CSPG & mouse antiMap2(1:80 and 1:200, respectively, Sigma-Aldrich, USA), rat anti-DSD-1 (1:200; SigmaAldrich, USA), rabbit anti-FGF8 (1:200; Thermo Fisher Scientific, USA), mouse anti-Tuj1 & mouse anti-HuC/D (both 1:400; Abcam, USA); rabbit anti-MMP2 & rabbit anti-MMP9 (both 1:100, Abcam, USA), mouse anti-Pax2 (1:50, Abcam, USA), goat anti-Doublecortin (1:200, Santa-Cruz Biotechnology, USA), mouse anti-Islt1/Lhx1/5/Laminin-C1/Tag1/HSPG (all .. Following washes, embryos were incubated for 2 h at RT with the following secondary antibodies; Goat anti-mouse Alexa488, Goat anti-mouse Alexa594, Goat anti-rabbit Alexa488, Goat anti-rabbit Alexa594 (1:300; diluted in blocking solution; Life Technologies, USA).



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